Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions

Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions

Blog Article

We present a novel liquid array diagnostics (LAD) method, which enables rapid and inexpensive detection of microbial markers in a single-tube multiplex reaction.We evaluated LAD both on pure Converter cultures, and on infant gut microbiota for a 15-plex reaction.LAD showed more than 80% accuracy of classification and a detection limit lower than 2% of the Illumina reads per sample.The results on the clinical dataset showed that there was a rapid decrease of staphylococci from 10-day- to 4-month-old children, a peak of bifidobacteria at 4 months, and a peak of Bacteroides in 2-year-old children, which is in accordance with findings described in the literature.

Being able to detect up to 50 biomarkers, LAD is a suitable method for assays motor belt where high throughput is essential.