Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions
We present a novel liquid array diagnostics (LAD) method, which enables rapid and inexpensive detection of microbial markers in a single-tube multiplex reaction.We evaluated LAD both on pure Converter cultures, and on infant gut microbiota for a 15-plex reaction.LAD showed more than 80% accuracy of classification and a detection limit lower than 2%